Bacterial CRISPR: accomplishments and prospects.
نویسندگان
چکیده
In this review we briefly describe the development of CRISPR tools for genome editing and control of transcription in bacteria. We focus on the Type II CRISPR/Cas9 system, provide specific examples for use of the system, and highlight the advantages and disadvantages of CRISPR versus other techniques. We suggest potential strategies for combining CRISPR tools with high-throughput approaches to elucidate gene function in bacteria.
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ورودعنوان ژورنال:
- Current opinion in microbiology
دوره 27 شماره
صفحات -
تاریخ انتشار 2015